FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 drought 2h 2.341000000081dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 drought 2h 2.341000000081dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15194653
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11900.024.013945
GACGGTC10850.021.7733257
TGCGTCG12550.018.28594225
GTCGCGC13150.017.2805124
CCTAGAC30750.017.269253
GAGCGCG18000.016.9993217
GCGTCGT14050.016.49384326
ACCGACG5700.016.1835678
GACAGTG11000.015.9539117
AGCGCGC15100.015.9430738
GGACTGC25150.015.8358696
CCGTGCG8300.015.72314559
TATACAC223000.015.56766245
GCCTAGT7300.015.4263871
ACTGTCA7450.015.40217244
ACGGTCC17600.015.3402988
GTACCGT17350.015.3034456
GTCTAGG30050.015.0649821
TAGCGTG5850.015.0002419
AGACCGA17350.014.9143736
Produced by FastQC (version 0.11.2)