FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 control 4h 1.3410000000937b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 control 4h 1.3410000000937b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14759527
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12650.022.0573225
GACGGTC11300.021.503597
GTCGCGC15300.018.38164124
CCTAGAC30350.017.4974563
GACAGTG11700.016.922427
CCGTGCG9350.016.8450669
CGTCGCG17000.016.80817223
TGCGTCG12450.016.2644325
ACTGTCC14750.016.1688638
GACTGTG7450.016.006097
GTCTAGG29850.015.9965681
ACGACTA8250.015.81762242
GTACCGT16700.015.3606646
GTCTATG31800.015.1573031
AGTACCG26200.015.0299785
TAAGGTG33750.015.0013495
TAGCCCG18550.014.79711918
GCGTCGT14150.014.78741326
TCGCGCG18950.014.60365525
CTAGACA37800.014.4060584
Produced by FastQC (version 0.11.2)