FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l02n01 control 2h 1.3410000000819a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l02n01 control 2h 1.3410000000819a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17435698
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12450.021.5078075
GACGGTC11650.019.8916137
GTCGCGC14900.017.96938324
TGCGTCG14750.017.84704225
GCGTCGT15600.017.30719226
GAGCGCG21400.016.611247
GTCTAGG35400.016.54251
AGCGCGC17500.016.4562428
GTACCGT20350.016.1438456
CCTAGAC33300.016.0148413
CCGTGCG11100.016.0134189
GGACTGC27250.015.5242226
TATACAC280650.015.46410245
GTCTATG37000.015.40103151
GGACCGT20600.015.0740656
ACTGTCC16950.014.999188
GACTGTG9350.014.9189697
TAAGGTG41750.014.8754855
GTGTATG49450.014.6207541
AGTACCG32250.014.4430575
Produced by FastQC (version 0.11.2)