FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 root 1.34200000009434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 root 1.34200000009434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17693383
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG4400.015.3401945
TAGGACA9100.015.08154
TATACCG8700.014.4820825
TCTAGAC4200.014.4613623
ATACACA748950.014.28834645
TATACAC1089950.014.10928945
TACACCG44850.013.9458385
CTAGACT5500.013.9082464
TCTGACG94200.013.51091145
CTATACT5850.013.4607214
TACACAT512000.013.39958545
GTTCTAG6100.013.2718391
CTACACT6550.013.0526424
ACACATC371950.013.02207745
TAACACG3355.456968E-1212.7604194
CACATCT267300.012.69431945
TACACTG6650.011.8415535
CCTACAC30400.011.6917143
TAGACAG5800.011.6373885
GTGTAGG34900.011.5985981