FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 heat recovery 4h 1.342000000093e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 heat recovery 4h 1.342000000093e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9905828
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG7150.021.3989015
GACGGTC7250.018.3101377
GTCTAGG20300.017.1716271
CCTAGAC18500.017.0235653
TGACGCT7350.016.21570445
GTACCGT12950.015.8106336
GTCTATG19350.015.1090841
CCGTGCG8800.015.0852859
AGTACCG19050.014.4096495
TATACAC136350.014.31567645
GTCTTAG18100.014.1645261
ACTGTCC9850.014.1622778
CTCACAC9550.013.8976973
TTAGGAC17300.013.7833253
CGTGTAC5200.013.4107353
GACTGTG4900.013.31617457
CCAGGGT8650.013.26323
GAGCGCG13500.013.1665177
GCCCTAG10600.013.1541151
ATAGACG10800.013.1244544
Produced by FastQC (version 0.11.2)