FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 heat 4h 2.342000000092e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 heat 4h 2.342000000092e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14793859
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG10250.020.6342725
TGACGCT10300.020.30226345
GACGGTC9350.018.769347
CCGTGCG8650.018.7290069
GTCTAGG28800.017.8050771
GTACCGT17250.017.086316
GACAGTG9800.016.7595677
TGCGTCG11850.016.33247225
TATACAC276650.016.25541545
GTCTATG31200.016.075031
ACTGTCC15500.015.8223028
GCGTCGT12950.015.64054626
AGTACCG25800.015.6105585
GTCGCGC12250.015.43127524
ATACACA183750.014.97796545
CCTAGAC29050.014.9456243
GAGCGCG17600.014.8289837
TAAGGTG32850.014.246665
GAGTGTC18500.014.229197
GGTCCGT17650.014.0232711
Produced by FastQC (version 0.11.2)