FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 heat 30 min 2.342000000093af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 heat 30 min 2.342000000093af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13041193
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG8850.020.3380875
GACGGTC9250.019.4586757
GTACCGT15700.017.9132926
GTCTAGG25600.017.8355331
GTCTATG27700.017.2953721
ACTGTCC13550.016.6047538
CCGTGCG8500.016.411679
CCTAGAC23950.015.6858973
ACCGTGC24550.015.0301768
TATACAC178500.014.55125845
GCACTGA14250.014.5257316
AGTACCG21300.014.3655645
AACAGAC13650.014.3400827
GGTCCGT15350.014.0716911
TACCGTG20900.013.7793497
TAAGGTG29100.013.6076475
ACTGCTC19200.013.5934128
ATACACA124350.013.52734745
GCCCTAG14500.013.4952621
ACGACTA8550.013.41551942
Produced by FastQC (version 0.11.2)