FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 drought recovery 4h 1.34200000009328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 drought recovery 4h 1.34200000009328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15809918
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11250.020.9995735
GACGGTC9850.019.8725017
TGACGCT13650.018.1219245
GTACCGT15950.017.2095176
TATACAC332050.016.6058545
TGCGTCG13650.015.8269625
CCTAGAC28350.015.6322763
GTCTAGG32250.015.55250551
TACCGTG21050.015.2845797
CCGTGCG11350.015.2645389
ACTGTCC15100.015.1984628
ATACACA213650.014.85143145
AGTACCG23550.014.8086145
GTCTATG30700.014.79921
GAGCGCG18850.014.323187
GACTGTG9150.014.26186857
GCGTCGT15600.013.70459326
GACAGTG13650.013.680917
GGACTGC24550.013.6553966
AGCGCGC17900.013.5752288
Produced by FastQC (version 0.11.2)