FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 drought 2h 3.342000000081f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 drought 2h 3.342000000081f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17328344
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT16400.020.84257545
TAGACGG11700.017.499695
TATACAC397250.017.1186545
CCGTGCG14950.015.501629
ATACACA253300.015.18142545
GTACCGT17050.014.91156
CTGACGC44450.014.16569545
GACGGTC11300.014.1366727
TATACTG45700.013.9822465
GACAGTG12950.013.8991277
TTATACA638350.013.75668445
GTCTAGG32550.013.6815731
TCTGACG47000.013.44610344
CCTAGAC29500.013.4215143
GTGTATG38400.013.3544331
TATACCG42750.012.9997695
GTCTTAG34100.012.9937241
ACTGTCC16800.012.9907288
GTCTATG31100.012.945371
GTCGCGC16200.012.77961724
Produced by FastQC (version 0.11.2)