FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 control 4h 2.342000000092c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 control 4h 2.342000000092c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17205190
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12050.019.6051855
GTACCGT19800.017.6129236
CCGTGCG11700.017.3075949
TATACAC277550.016.17301645
GACGGTC11900.015.692647
GTCTATG36250.015.3873331
GTCTAGG34200.015.1916711
AGTACCG30050.015.1243115
GACAGTG12500.015.1193877
GACTGTG11700.014.99939257
CCTAGAC33950.014.8416523
ACTGTCC18350.014.7133448
ATACACA187650.014.62119845
TACCGTG25600.014.5892527
TGCGTCG14350.014.42731325
ACCGTGC28300.014.3104168
TGACGCT11700.014.22386445
CTAGACA45300.014.2045984
GAGCGCG20300.014.1866177
GCGTCGT15200.014.06495826
Produced by FastQC (version 0.11.2)