FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 control 30min 2.342000000092a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 control 30min 2.342000000092a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13511799
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9400.021.5421435
TGACGCT11000.017.99006345
GTCTAGG28450.017.3899841
CCGTGCG8800.017.1311829
GACGGTC9900.017.0451327
CCTAGAC23050.016.4930113
GAGCGCG14400.016.4059377
TGCGTCG10500.016.2881825
GCGTCGT11000.015.95743526
TATACAC237050.015.91822745
GTACCGT14800.015.3544386
AGTACCG20900.015.2867915
ATACACA157800.015.21972945
GCCTAGT6700.014.7685081
GTGCGAG10800.014.58386611
GTATAAG50200.014.4248371
GTCTATG28850.014.4206441
AGCGCGC13900.014.4065748
GGTCCGT13600.014.22846111
ACTGTCA6400.014.05629644
Produced by FastQC (version 0.11.2)