FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 control 30 min 3.3420000000936b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 control 30 min 3.3420000000936b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12699060
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9850.021.014265
GACGGTC9200.018.585887
GTCTAGG25750.018.0812721
CCTAGAC23350.017.4378363
TGCGTCG10800.017.29516225
CCGTGCG8700.016.0343139
GCGTCGT12500.015.303575526
GTACCGT13700.014.9443916
TATACAC185150.014.61129945
GACTGTG6350.014.5267187
GTCTATG26650.014.3478761
TTGCGTC12900.014.30476824
GTCGCGC12450.014.27954624
GAGCGCG16550.014.1381547
GGTCCGT13150.013.85927811
AGCGCGC14900.013.74086958
ACTGTCA8150.013.52160344
CTAGACC16600.013.4175824
GCACTGA13250.013.4143186
ATACACA131700.013.38681445
Produced by FastQC (version 0.11.2)