FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n02 control 2h 3.342000000081b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n02 control 2h 3.342000000081b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19227791
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT17800.020.46569845
TAGACGG12900.019.1851445
TATACAC405600.017.31987245
TGCGTCG14150.016.69928225
GACGGTC12600.016.2492377
GTACCGT19850.015.8682726
GTCTAGG36800.015.6459821
GACAGTG14250.015.3150687
GTCTATG38750.015.2649261
CCGTGCG13700.015.1098019
GAGCGCG20700.014.9992947
CCTAGAC33150.014.9969533
AGCGCGC17600.014.8293118
GCGTCGT16150.014.63152626
ATACACA256800.014.58853145
GGACCGT26350.014.0031366
TATACCG49100.013.7009845
TACCGTG26450.013.6950087
GGACTGC29150.013.661456
AGTACCG29800.013.5144655
Produced by FastQC (version 0.11.2)