FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 root 1.34100000009437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 root 1.34100000009437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17693383
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG9900.014.7761485
ATACACA753700.014.34305545
TATACAC1095150.014.14210445
TCCGAGC65500.013.97976645
CTCCGAG79000.013.4988945
TACACCG48350.013.4519245
TACACAT515350.013.35875445
ACACATC372600.012.93975645
TATACTG4100.012.6248735
CACATCT270100.012.61095845
GTATTAT3701.8189894E-1212.174271
TCTCCGA107000.012.11115445
TCTACAC28150.011.832193
CTAGACT4800.011.721434
GTGTAGA29250.011.6269481
GTGCTAG8950.011.5757581
GTCTAGA4300.011.5230881
CCTACAC35800.011.5040263
TTATACA1468550.011.45627845
GTCTTAG3753.6379788E-1111.4113481
Produced by FastQC (version 0.11.2)