FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 heat recovery 4h 1.341000000093e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 heat recovery 4h 1.341000000093e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9905828
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG7250.021.7300075
GACGGTC7250.019.2408477
CCTAGAC20350.018.9117413
ACGACTA4700.018.18997442
GACTGTG5150.017.9121257
GTCTAGG18700.016.8636051
GACAGTG7450.016.3082737
ACTGTCC12050.015.6842138
GCCCTAG9800.015.3997181
GTACCGT10200.015.0035236
TAGACGC6050.014.8800535
ACTGCTC13950.014.83838
GTCTATG20100.014.7925111
AGTACCG16200.014.7262015
ACAGTGA9050.014.4232766
CTAGACG9300.014.278054
TATACAC136150.014.22732145
CCGTGCG8500.014.0290229
GGACTGC16050.014.0219846
AACAGAC10900.013.6234757
Produced by FastQC (version 0.11.2)