FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 heat 4h 2.341000000092e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 heat 4h 2.341000000092e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14793859
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11900.025.1525125
GACGGTC9600.023.9048867
CCGTGCG7900.019.9355729
GTACCGT15200.018.2105676
TGCGTCG12700.018.0711825
CCTAGAC27550.017.8895423
GTCGCGC13450.017.06349424
ACTGTCC15750.016.4276358
ACGGTCC17150.016.3984818
GCGTCGT14000.016.39319826
GACAGTG11400.016.3806467
TATACAC278250.016.33269345
AGCGCGC15550.016.0601798
AGTACCG24950.015.9653855
GGACTGC22200.015.9150756
GTCTAGG31150.015.6905531
CTAGACA35700.015.5075644
GTCTATG31100.015.498511
ACTGAGC33200.015.31539258
GAGCGCG17650.015.2965787
Produced by FastQC (version 0.11.2)