FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 heat 30 min 2.341000000093a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 heat 30 min 2.341000000093a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13041193
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9650.023.0861915
GACGGTC9850.021.0148417
GTACCGT14450.018.3761836
CCGTGCG10300.018.1307339
GTCTAGG26250.017.4187131
CCTAGAC25550.017.174663
ACTGTCC14100.015.9571518
AGTACCG20650.015.8012855
TGCGTCG11350.015.26450325
ACCGTGC22250.014.8648948
TATACAC176950.014.63437845
ACTGCTC19150.014.4514318
GCCCTAG12650.014.422611
GACAGTG11300.014.3360197
ACGGTCC14750.014.1861788
GTCTATG27950.014.1833941
ACGACTA7350.014.08088642
ACTAGGC8050.013.9771473
GTACACG9350.013.972231
GCGTCGT12800.013.88699426
Produced by FastQC (version 0.11.2)