FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 drought recovery 4h 1.3410000000932b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 drought recovery 4h 1.3410000000932b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15809918
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12250.021.3109865
GACGGTC11800.020.0201237
CCGTGCG11150.018.5640349
GTACCGT16150.018.2543056
TATACAC329400.016.80174645
AGTACCG24650.015.885985
ATACACA214400.014.97414845
GTCTATG31050.014.9436631
GACTGCG8550.014.7360587
GTCGCGC14600.014.33215424
GCCCTAG13150.014.2168631
TACCGTG22200.013.9857437
GTCTAGG27250.013.9692021
GGACTGC24050.013.9424076
ACTGAGC35150.013.8257238
ACTGTCC13850.013.807938
CACTGAG39300.013.7969767
CCTAGAC28300.013.7575583
ACGGTCC20200.013.5883878
GTGCGAG12950.013.55140311
Produced by FastQC (version 0.11.2)