FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 drought 2h 3.341000000081f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 drought 2h 3.341000000081f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17328344
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11900.018.9125735
TATACAC400750.017.0102745
CCGTGCG14450.016.3488549
GTACCGT17700.015.6394186
CCTAGAC30650.015.4199363
ATACACA256750.015.05410145
GACTGTG10150.014.6299837
ACTGTCC15850.014.6208938
TGCGTCG13500.014.50003125
GACGGTC12750.014.2935727
GTCTAGG33500.013.7840011
AGCCGTG15850.013.769197
TTATACA641050.013.75388745
ACCGTGC29450.013.5988028
TACCGTG26250.013.542347
AGCGCGC18150.013.3879188
GCGTCGT15000.013.20002826
TAAGGTG33200.013.0832835
CACTGAG39950.013.0658347
AGTACCG27300.013.0254275
Produced by FastQC (version 0.11.2)