FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 control 4h 2.341000000092cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 control 4h 2.341000000092cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17205190
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13100.019.413575
GTCTAGG36050.018.5582071
GACGGTC12300.018.4746827
CCTAGAC32400.018.0603163
GCGTCGT15700.017.48487526
TGCGTCG15600.017.16424625
CCGTGCG12050.016.9908479
GTACCGT19050.016.4214136
TATACAC277500.016.26324545
GAGCGCG21100.015.9944567
AGCGCGC19200.015.8195188
GACAGTG12950.015.4625437
GTCGCGC16400.015.36655624
GGACCGT23500.014.7483566
ATACACA187500.014.68653745
ACTGTCC17350.014.52376658
CTAGACA44550.014.29674
GCCTAGT8200.014.2848051
GCCCTAG18150.014.2726961
TTGCGTC18750.014.16064724
Produced by FastQC (version 0.11.2)