FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 control 30min 2.341000000092a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 control 30min 2.341000000092a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13511799
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9400.019.8713025
CCGTGCG8600.018.8365279
CCTAGAC24500.018.003883
GTCGCGC11550.017.33748424
GCGTCGT10450.017.22470326
GTACCGT14250.016.8981296
GACGGTC9200.016.874397
TGCGTCG10550.016.84816725
ACTGTCA7350.016.83581444
GCCTAGT6550.016.5062791
TATACAC237000.016.04295245
GTCTAGG26750.015.914291
GTGCGAG10300.015.72758711
ACGACTA7500.015.5990942
GTCTATG29650.015.3453181
GAGTGTC18200.015.3291157
ATACACA156850.015.23301645
AACAGAC16600.014.5024877
TAAGGTG28650.014.4533765
GAGCGCG16050.014.438737
Produced by FastQC (version 0.11.2)