FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 control 30 min 3.3410000000936e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 control 30 min 3.3410000000936e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12699060
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9950.021.2612785
GACGGTC9850.020.7858757
CCTAGAC26850.018.5239073
GTCTAGG25150.017.5535011
CCGTGCG9500.017.2887029
TGCGTCG10650.016.05644425
GACAGTG10100.015.8161277
GTCGCGC13200.015.68192224
GTACCGT13950.015.1642576
GAGTGTC16200.015.1382167
GCGTCGT12200.014.93874226
CTAGACA34100.014.9155294
TATACAC186400.014.56819445
TAAGGTG31750.014.389215
AACAGAC14700.014.3871157
AGTACCG21750.014.3826915
TAGCCCG14850.014.24100518
ACTGTCA6950.013.9198644
CTAGACC21450.013.8494094
GTCTATG27750.013.79849151
Produced by FastQC (version 0.11.2)