FastQCFastQC Report
Fri 1 May 2015
H3KYGBCXX l01n01 control 2h 3.341000000081b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KYGBCXX l01n01 control 2h 3.341000000081b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19227791
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13500.022.004935
GACGGTC13450.018.7353367
CCGTGCG13400.017.4620139
TATACAC405650.017.30412745
TGCGTCG12900.016.74423625
GTACCGT19700.016.5643656
GTCTAGG35850.015.8331181
GACAGTG13700.015.437367
ACCCTGG17600.015.22962951
CCTAGAC36450.014.7563933
ATACACA254300.014.72154945
GTCGCGC16650.014.45950324
GTGCGAG14850.014.39338311
ACCGTGC32700.014.3113758
AGCGCGC16950.014.20299158
TAAGGTG40800.014.175975
GACTGTG9450.014.0470777
CTAGACG12500.013.8631064
GAGCGCG20200.013.8113487
ACTGTCC17300.013.7855958
Produced by FastQC (version 0.11.2)