FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 root 2.3410000000942a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 root 2.3410000000942a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19788437
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA869200.014.44564845
TATACAC1266200.014.19574845
CTCCGAG82800.014.0230345
TACACCG52450.013.8125795
TACACAT590800.013.71148745
TATACTG5300.013.5843385
TATACAG5400.013.3327765
TAGACCG22400.012.9570475
ACACATC423000.012.74585545
TCCGAGC67800.012.74453645
CACATCT308300.012.66334645
TCTCCGA113800.012.496757545
TAGGACA11050.012.2166844
TACACAG7000.012.2137765
TAGACAG6350.012.04674155
TTAGACA3803.6379788E-1211.841614
TTATACA1705900.011.5735545
CTTAGAC5700.011.446893
GTCTTAG4750.011.3805991
TCTAGAC4350.011.3788363