FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 heat recovery 4h 2.341000000092f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 heat recovery 4h 2.341000000092f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13983663
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG9500.021.7884085
GACGGTC10150.017.7331227
CCGTGCG9100.017.0596059
GACAGTG10200.015.8815757
TATACAC313000.015.80956145
GTCTAGG27950.015.6354181
GTACCGT13700.015.43720156
TGCGTCG12300.015.365211525
ATACACA208200.015.3045845
CCTAGAC29200.015.1023
ACTGTCC13950.014.9992668
TAAGGTG28500.014.4466615
GAGCGCG15700.014.0438997
CTTAGAC25450.013.9678823
AACAGAC16750.013.8351437
GACTGTG8200.013.7188417
GCGTCGT13450.013.54962126
GACTGCG7150.013.5308077
AGCGCGC14850.013.3326828
AGTACCG21100.013.2221015
Produced by FastQC (version 0.11.2)