FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 heat 4h 3.341000000093be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 heat 4h 3.341000000093be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11010274
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG8900.021.2349055
CCTAGAC20550.017.1889323
ACTGTCC12850.016.9835998
GACAGTG9050.016.9052437
CCGTGCG12700.015.5897829
GTACCGT13350.015.3363226
GTCTAGG20300.015.3155381
GTCTATG22650.015.218521
TATACAC142600.014.80129245
GGACTGC17250.014.7384046
GACGGTC12150.014.6289087
GGACCGT14950.014.5979416
AGTACCG20100.014.4395875
ACTGTCA6450.014.30343844
GTGTGAG9350.014.21641
GAGTGTC14150.014.1512457
TATACTG29900.013.9207175
TTAGGAC21150.013.7227273
ACAGTGA9900.013.6356916
GTGTTAC5450.013.2282591
Produced by FastQC (version 0.11.2)