FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 heat 30 min 3.34100000009338.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 heat 30 min 3.34100000009338.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13569447
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11800.019.0668585
CCTAGAC25000.016.4691893
GTCTAGG25250.015.8817911
GACGGTC14450.015.2587637
TATACAC234200.015.12306445
GTACCGT16350.014.7240466
GACAGTG11500.014.2819067
AGTACCG22850.014.1787335
CACTGAG36650.013.9965837
ATACACA160550.013.8193845
GGACTGC22800.013.7164316
GGACCGT18400.013.6949786
GACTGTG7100.013.626097
GTCTATG28150.013.6054011
ACCGTGC32850.013.5609768
CTAGACA33250.013.5331684
TAGCCCG15550.013.45592918
GAGTGTC18450.013.4139757
ACTGAGC32700.013.3479668
ACTGTCC15100.013.11193758
Produced by FastQC (version 0.11.2)