FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 heat 2h 3.3410000000823c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 heat 2h 3.3410000000823c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15070661
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12900.023.0222365
GACGGTC11950.022.4048657
CCGTGCG10050.019.924499
GTACCGT16600.019.6527446
TGCGTCG12450.017.52945925
GTCTAGG30750.017.2145791
GTCGCGC15300.017.2051224
GAGCGCG16150.016.9961517
GCGTCGT13650.016.6477826
CCTAGAC30150.016.4171833
CGTCGCG16200.016.38816323
TATACAC257750.016.12471445
AGTACCG27250.016.017645
ACTGTCA8650.015.86856144
ACTGTCC15000.015.4493168
GACAGTG12800.015.1165187
AGCGCGC15450.014.9993358
ATAGACG18800.014.9594444
CGTGCGA14150.014.94633510
GTCTATG32250.014.7375871
Produced by FastQC (version 0.11.2)