FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 heat 2h 1.34100000008212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 heat 2h 1.34100000008212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17578518
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG9650.024.9471269
GACGGTC11400.019.5386437
TAGACGG13350.018.5385385
TGCGTCG13350.018.20162825
GCGTCGT13500.018.16604826
GTCGCGC13700.017.900824
AGCCGTG15100.017.5820337
GTACCGT18200.017.3069576
CCTAGAC31150.016.9735953
GAGCGCG18050.016.9521877
GTCGTGC5350.016.8217148
GACTGTG8550.016.5782437
GACAGTG12950.016.3313527
GTGCGAG13450.016.05879811
TATACAC319550.016.04065945
AGCGCGC16800.015.9368218
GTCTAGG34500.015.9323521
GTCTATG36400.015.1626051
TAAGGTG38450.015.1554035
AGTACCG28700.014.8948365
Produced by FastQC (version 0.11.2)