FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 drought recovery 4h 2.341000000093f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 drought recovery 4h 2.341000000093f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12562690
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11000.019.8399285
CCTAGAC23950.017.2851583
GACGGTC11150.017.1516197
GTACCGT13500.015.6658936
CCGTGCG11950.015.4385679
ATACACA158300.015.09569445
TATACAC229250.014.8504345
GAGTGTC17250.014.4775467
GTCTAGG26700.014.1755041
TAAGGTG28000.014.1421585
GACAGTG11050.014.0490797
TAGAGGT26900.013.96771054
ACTGTCC12750.013.9404878
TACACAT99850.013.7464745
CTAGACA32800.013.5130514
AGACACT18500.013.4993326
GGACTGC19900.013.454116
AACAGAC14550.013.4529447
ACACCGC19050.013.3457986
GGACCGT17950.013.2862516
Produced by FastQC (version 0.11.2)