FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 drought 2h 2.341000000081dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 drought 2h 2.341000000081dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14814765
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11550.026.6870385
GACGGTC11200.025.311297
CCGTGCG8350.021.8253069
TGCGTCG12100.018.03669525
GACAGTG14100.018.0310547
GTACCGT18000.016.9991876
GTCTAGG28450.016.9468421
GCGTCGT13300.016.7476626
ACGGTCC18700.016.3628548
GAGCGCG16500.016.2264987
AGTACCG28100.016.013475
ACTGTCC14350.015.9922688
AGACGGT18350.015.9392946
TAAGGTG37500.015.89924055
AGCGCGC14750.015.8636488
GTCGCGC13650.015.65859224
GTGCGAG11050.015.47437311
ACCGTGC24900.015.451078
GTCGTGC3950.015.3790128
GCCTAGT6750.015.3536391
Produced by FastQC (version 0.11.2)