FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 control 4h 1.3410000000937b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 control 4h 1.3410000000937b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14342731
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12400.022.4987965
GACGGTC11100.022.2961037
CCGTGCG9100.020.273649
TGCGTCG12650.018.49735625
GCGTCGT12900.017.96459226
CCTAGAC28550.017.2582723
GTACCGT18150.017.1065226
GACAGTG11750.017.0416417
GTCTATG30400.016.5991251
GAGCGCG18300.016.5974717
GTCTAGG27600.016.2425691
AGCGCGC16700.015.8973528
GTGCGAG10350.015.86871511
ACTGTCC17250.015.6513358
AGTACCG25900.015.4624935
GGTCCGT18500.015.08027511
TTGCGTC15900.014.85774724
AACAGAC17300.014.8257967
TATACAC195850.014.68397945
GGACTGC24400.014.4766846
Produced by FastQC (version 0.11.2)