FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l02n01 control 2h 1.3410000000819a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l02n01 control 2h 1.3410000000819a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16942775
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGTC11500.022.694557
TAGACGG13500.022.165595
TGCGTCG13250.017.9993925
GAGCGCG18150.016.8586857
GCGTCGT14150.016.85460326
GTCTAGG31450.016.8321481
CCGTGCG10600.016.55589
GACAGTG12700.016.4755787
CCTAGAC33150.016.356673
GTCGCGC14900.016.15693324
GTACCGT18950.016.0282466
TATACAC281650.015.84280445
AGCGCGC16700.015.6279838
ACTGTCC17400.015.5164878
AGACCGA19050.015.1173756
AACAGAC20300.014.8514967
ACTGTCA9950.014.69973144
GTGCGCT12400.014.69686711
CGTCGCG16700.014.41546623
GGACTGC26400.014.3174866
Produced by FastQC (version 0.11.2)