FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 root 1.34100000009437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 root 1.34100000009437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17367122
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG4300.015.1733745
TTAGACT4050.014.9989674
ATACACA722800.014.46678945
TATACAC1056300.014.234323545
GTGTTAG3250.013.8657991
TCCGAGC64750.013.76173145
TACACCG46500.013.6442225
TACACAT491550.013.33034345
CTCCGAG78650.013.24646345
ACACATC357250.013.06333745
TGTATAA6350.012.75502714
TCTACAC25450.012.4647613
TAGACAG6050.012.2718825
CACATCT255950.012.14104845
GTGTAGG34450.011.9036581
TAGCACT8000.011.8116874
CTAGACA3451.05501385E-1011.7383234
GTTCTAG5200.011.6992671
GTCCTAT8100.011.683221
GTGTATA6950.011.65387313
Produced by FastQC (version 0.11.2)