FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 heat recovery 4h 1.341000000093e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 heat recovery 4h 1.341000000093e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9570083
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG7900.021.9290225
GACGGTC7500.019.7987757
ACTGTCC9150.018.1955958
GTCTAGG19650.017.2012921
GTACCGT12900.016.9175596
CCTAGAC16950.016.724633
CCGTGCG8000.016.0302589
GGACTGC16050.015.980326
AGTACCG17750.015.5905855
GACTGTG4200.015.5347537
GACTGCG6000.015.3740487
AACAGAC11450.015.1300687
GACAGTG8550.014.2096467
GTCTATG20750.014.1174931
CTAGACA24600.014.0844944
ACCCTGG9150.014.0373811
TGCGTCG9700.013.916882525
CTAGACC13300.013.7021594
TATACAC133050.013.64799245
ACCGTGC19750.013.5561238
Produced by FastQC (version 0.11.2)