FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 heat 4h 2.341000000092e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 heat 4h 2.341000000092e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14225843
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG10200.022.9397965
GACGGTC9500.022.4986467
CCGTGCG9800.019.9732869
TGCGTCG11800.019.2574525
CCTAGAC29050.018.9747623
GTACCGT14600.018.1838386
GCGTCGT13450.016.89542826
AGTACCG23150.016.7160575
GAGCGCG18150.016.6105697
GTCGCGC13150.016.59619124
GTCTAGG31900.016.1765921
TATACAC265750.015.89225645
AGCGCGC15750.015.8561888
GTGCGAG12350.015.48489711
TATACTG44050.015.4247245
GTCTATG31000.015.4104891
ACTGTCC14800.015.3538068
AGCCGTG11650.015.2565947
TTGCGTC15300.014.99930824
GACTGTG8000.014.9053537
Produced by FastQC (version 0.11.2)