FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 heat 30 min 2.341000000093a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 heat 30 min 2.341000000093a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12591546
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG10000.021.8234185
GACGGTC9500.019.4196477
CCTAGAC25900.016.4177283
GTACCGT15450.016.1638776
GACAGTG10000.015.5238757
ACTGTCA6950.015.21605444
GAGTGTC17150.015.0863717
CCGTGCG9450.014.9989159
CTAGACA33250.014.7508114
TATACAC172700.014.69674945
TACCGTG19450.014.6904547
AGTACCG23250.014.6118445
GTCTAGG24650.014.44236851
ACCACGC10000.014.3989568
ACTGTCC13450.014.3855748
ATAGACG13950.014.031244
TATACTG37300.013.8729895
AACAGAC15950.013.823457
GCGTCGT12600.013.74965926
GTCTAGA26900.013.7369341
Produced by FastQC (version 0.11.2)