FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 drought recovery 4h 1.3410000000932b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 drought recovery 4h 1.3410000000932b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15226046
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12100.019.3377325
CCTAGAC27200.018.6110633
CCGTGCG12150.017.9619319
GACGGTC11650.017.960347
GTACCGT15100.016.836786
GACTGTG9000.016.7490377
GTCTAGG27500.016.7146511
TATACAC329000.016.70151345
ACTGTCC13800.015.9773448
GACAGTG11850.015.1890017
CTAGACG11000.014.9309614
ATACACA213400.014.86725445
GGACTGC23300.014.6772686
TAGACGC8900.014.662085
GTGCGAG13900.014.56750811
AGTACCG24600.014.450395
TAAGGTG30100.014.4260835
TGCGTCG13300.013.87161225
TACCGTG22000.013.80602557
GTCCTAA31000.013.7372541
Produced by FastQC (version 0.11.2)