FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 drought 2h 3.341000000081f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 drought 2h 3.341000000081f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16736588
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13050.018.4471265
TATACAC393150.017.2898845
GTACCGT16300.015.8732446
CCTAGAC30650.015.5618363
CCGTGCG14550.015.1536959
GACAGTG14250.014.8411817
GTCTAGG32200.014.5558671
ATACACA251500.014.36835745
GACTGTG9200.014.1838997
GACGGTC12050.014.0032767
AGTACCG26350.013.9175395
CTAGACA36450.013.6411254
CACTGAG40300.013.5661527
GGACTGC25050.013.5620296
TATACTG44100.013.5195655
TTATACA624100.013.42986445
TAAGGTG35450.013.3278015
AGACCGA18600.013.3056216
ACTGTCC16000.013.2179268
GTCGCGC15850.013.20142524
Produced by FastQC (version 0.11.2)