FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 control 4h 2.341000000092cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 control 4h 2.341000000092cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16627456
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13350.020.5601335
GACGGTC12650.018.3186767
GTACCGT19300.017.9519146
AGTACCG27800.016.4285285
CCTAGAC32550.016.3120423
GACTGTG8550.016.0513327
TATACAC278450.016.03262145
CCGTGCG11650.016.0287469
GGACTGC27800.015.9429556
TGCGTCG12350.015.84963525
GTCTAGG32350.015.8105571
GACAGTG13450.015.5563627
ACTGTCC16450.015.1811438
ATACACA187850.014.85321745
TTAGGAC32500.014.67573453
GAGTGTC22200.014.3907277
ACCGTGC28800.014.1394818
GTCTATG34150.014.0534991
TAAGGTG39350.014.0077625
GAGCGCG19450.013.9962987
Produced by FastQC (version 0.11.2)