FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 control 30min 2.341000000092a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 control 30min 2.341000000092a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13035164
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG11000.025.1575415
GACGGTC9050.024.611747
CCGTGCG8600.018.3128249
CCTAGAC23500.017.232983
GTCGCGC11250.016.99947524
TGCGTCG9350.016.84433425
GTACCGT16150.016.4386166
TATACAC233600.016.3173545
GAGCGCG14250.016.2621567
GACTGTG6750.015.9990157
GTCTAGG25100.015.9795261
AGTACCG23950.015.96978955
GTCTATG26950.015.8859221
GAGTGTC15950.015.5162857
GCCCTAG13050.015.3672841
ATACACA151900.015.28730845
ACTGTCA7200.015.00051444
GCGTCGT11050.014.86379326
AGACGGT16850.014.8210446
ACTGTCC12600.014.8205168
Produced by FastQC (version 0.11.2)