FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 control 30 min 3.3410000000936e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 control 30 min 3.3410000000936e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12267426
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG8950.020.6130925
GTCTAGG24500.017.6618061
CCGTGCG9100.017.5537269
GTACCGT14500.017.3781036
GACGGTC9550.016.9621737
CCTAGAC27650.016.1923663
GACTGTG7550.016.0915977
GACAGTG9950.015.8280457
AGTACCG20850.015.6463965
TATACTG35600.015.4834765
AGCCGTG10950.014.9989587
TATACAC175650.014.82175245
ACTGTCC12000.014.6239838
ACACCGC15400.014.6093756
GGACTGC18900.014.52279956
CGTGTAC6150.014.2673023
ACTGCTC17700.014.23629958
GTCGCGC11250.014.19936124
GCGTCGT10350.014.12997226
GCCCTAG12800.013.9096791
Produced by FastQC (version 0.11.2)