FastQCFastQC Report
Fri 1 May 2015
H3KWGBCXX l01n01 control 2h 3.341000000081b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KWGBCXX l01n01 control 2h 3.341000000081b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18538043
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG12300.019.9376135
GTACCGT19350.017.5568966
CCTAGAC34600.016.8412913
TATACAC392650.016.6874645
GACGGTC12000.016.6863177
GTCGCGC16100.016.62970524
CCGTGCG13200.015.3398239
TACCGTG29400.015.0754627
TGCGTCG14400.014.99938225
GGACTGC29400.014.9989376
ATACACA251800.014.98586545
GTCTAGG35200.014.9811761
AGTACCG30100.014.9491065
GACTGTG11150.014.3263397
ACTGTCC16650.014.323318
CTAGACA44100.014.2847024
ACCGTGC30600.014.1166478
TATACTG52050.014.0912215
GGACCGT23950.013.9969216
TATACCG49950.013.8728915
Produced by FastQC (version 0.11.2)