FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28261778
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4373071.5473442612138557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1019430.36070978973792805No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT666610.2358698026712969No Hit
GGGCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA352190.12461707115525428No Hit
GCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA331220.1171971558194251No Hit
CGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA329190.1164788712160997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG92850.054.1657311
ACGTCTG91350.053.6356915
CACACGT96100.052.22538412
CACGTCT97000.051.0901814
ACACGTC99950.050.0379113
AGCACAC107600.047.52131710
GTACTCT161500.046.604833
TACTCTA167800.045.847724
CAGTCAC93000.042.74899327
TCTAGTT178450.042.6168567
ACTCTAG196500.042.4619145
CCAGTCA98400.041.57676726
TCGGAAG123250.041.41293
CTCTAGT190650.040.442666
TGTACTC188050.039.914322
CGTCTGA122650.039.89084616
CTGAACT125950.039.37305519
TTGTACT183250.039.1411631
AGAGCAC136700.037.3283428
CTCCAGT120050.037.28534324
Produced by FastQC (version 0.11.5)