FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-9R-LFY.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-9R-LFY.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18309444
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT754970.41233911854450633No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC227810.1244221288205147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA156550.043.2186284
GTACTCT158100.042.4847343
TCTAGTT176950.038.5508967
ACTCTAG224250.037.0545545
TGTACTC197800.034.0813982
CTCTAGT209850.033.9098976
TTGTACT201550.033.3614581
CTAGTTG215750.031.1967958
ACCTACT194750.030.64154233
ACACCTA196550.030.62828431
TAGTTGT223350.030.4644269
CTAATGC219200.029.98601521
GTTACCT227050.029.25846114
ATGCTGG225900.028.83334524
TGGACAC224900.028.80608228
TACCTCT231800.028.59787816
TGTTACC236400.028.24929613
ACCTCTA239550.027.76018317
TCAGGAC205550.027.75445260
TCTAATG238350.027.70897520
Produced by FastQC (version 0.11.5)