FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-9G-WRKY33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-9G-WRKY33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17932312
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1562540.8713544578077829No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC353070.19689039539352204No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG221310.12341409183601088No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG216770.12088234913601771No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT210690.1174918214673044No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA192820.10752656991468808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG102700.053.91607711
ACGTCTG103900.053.08991215
CACACGT110200.050.21587412
TACTCTA278450.049.300344
ACACGTC113950.048.56222513
GTACTCT283250.048.415593
CACGTCT115200.048.0049114
AGCACAC117300.047.205310
ACTCTAG316650.047.1886375
CACTACA118200.046.22208431
CTACAGC118650.046.13514733
ACTACAG122150.044.7560432
TCTAGTT308550.044.512147
CTCTAGT312050.044.3071026
GAGCACA128050.043.6252569
TACAGCA123900.043.55880434
TCCAGTC128300.042.77447525
GTCACTA129600.042.6424829
AGTCACT130400.042.21982628
CAGTCAC129900.041.95123727
Produced by FastQC (version 0.11.5)