FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-8R-MYB4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-8R-MYB4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16805120
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1386700.8251651877523041No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC331660.19735651991773934TruSeq Adapter, Index 7 (97% over 37bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC301820.17960002665854216No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG201280.11977302155533552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA230750.050.8901334
GTATGCC58150.050.859345
CGTATGC58400.050.8213744
GTACTCT231750.050.5950243
TATGCCG62000.047.81446
GCACACG65100.047.2593811
TCTAGTT254700.045.9664427
TCGTATG64500.045.96074743
CTCGTAT62000.044.99144742
ACGTCTG69100.044.6240515
ATGCCGT67700.043.8447
CACACGT72300.042.6505112
AGCACAC73300.041.924910
TTGTACT281250.041.5047381
TGTACTC283050.041.3262522
GCCGTCT71650.041.1300549
ACTCTAG316150.040.83015
CACGTCT76500.040.0793114
CTCTAGT311100.039.2433136
TAGTTGT305600.038.2079859
Produced by FastQC (version 0.11.5)