FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-8G-GBF2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-8G-GBF2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15568783
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT2582631.658851562129166No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC1549820.9954663765305226TruSeq Adapter, Index 3 (97% over 36bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC767080.4927038934257096No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG398580.25601230359495664No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT366070.23513077419089215No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG362250.23267714631259231No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA323050.20749855656668864No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291160.18701525995962562No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA246840.15854803808364468No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT227720.14626705247288757No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA200760.1289503489129497No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT187980.12074161480701477No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA185320.11903306764568561No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA184180.1183008331479731No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA181720.11672074817922506No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA181520.11659228598664391No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT178140.11442127493202263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC211150.063.8828245
CGTATGC213250.063.48350544
TATGCCG213350.063.22408346
GCACACG220850.062.7265811
ACGTCTG226500.061.19297415
TCGTATG219900.060.94296643
CTCGTAT203650.060.6499942
CACACGT228600.060.55467612
ACACGTC231250.059.92148213
AGCACAC231500.059.91609610
CACGTCT232000.059.81790514
ATGCCGT231400.058.35288647
GCCGTCT230350.057.99591449
CTAATCG237300.057.47727233
TCCAGTC239600.057.290925
CAGTCAC238700.057.21365727
CCAGTCA239450.057.0490726
AGTCACT239800.056.965828
GAGCACA245500.056.5278059
GTCACTA243900.056.00819829