Basic Statistics
Measure | Value |
---|---|
Filename | H3KMLBGXC_n01_0801-8G-GBF2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15568783 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 258263 | 1.658851562129166 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC | 154982 | 0.9954663765305226 | TruSeq Adapter, Index 3 (97% over 36bp) |
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC | 76708 | 0.4927038934257096 | No Hit |
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG | 39858 | 0.25601230359495664 | No Hit |
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT | 36607 | 0.23513077419089215 | No Hit |
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG | 36225 | 0.23267714631259231 | No Hit |
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA | 32305 | 0.20749855656668864 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 29116 | 0.18701525995962562 | No Hit |
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA | 24684 | 0.15854803808364468 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 22772 | 0.14626705247288757 | No Hit |
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA | 20076 | 0.1289503489129497 | No Hit |
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT | 18798 | 0.12074161480701477 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 18532 | 0.11903306764568561 | No Hit |
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA | 18418 | 0.1183008331479731 | No Hit |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 18172 | 0.11672074817922506 | No Hit |
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA | 18152 | 0.11659228598664391 | No Hit |
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT | 17814 | 0.11442127493202263 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATGCC | 21115 | 0.0 | 63.88282 | 45 |
CGTATGC | 21325 | 0.0 | 63.483505 | 44 |
TATGCCG | 21335 | 0.0 | 63.224083 | 46 |
GCACACG | 22085 | 0.0 | 62.72658 | 11 |
ACGTCTG | 22650 | 0.0 | 61.192974 | 15 |
TCGTATG | 21990 | 0.0 | 60.942966 | 43 |
CTCGTAT | 20365 | 0.0 | 60.64999 | 42 |
CACACGT | 22860 | 0.0 | 60.554676 | 12 |
ACACGTC | 23125 | 0.0 | 59.921482 | 13 |
AGCACAC | 23150 | 0.0 | 59.916096 | 10 |
CACGTCT | 23200 | 0.0 | 59.817905 | 14 |
ATGCCGT | 23140 | 0.0 | 58.352886 | 47 |
GCCGTCT | 23035 | 0.0 | 57.995914 | 49 |
CTAATCG | 23730 | 0.0 | 57.477272 | 33 |
TCCAGTC | 23960 | 0.0 | 57.2909 | 25 |
CAGTCAC | 23870 | 0.0 | 57.213657 | 27 |
CCAGTCA | 23945 | 0.0 | 57.04907 | 26 |
AGTCACT | 23980 | 0.0 | 56.9658 | 28 |
GAGCACA | 24550 | 0.0 | 56.527805 | 9 |
GTCACTA | 24390 | 0.0 | 56.008198 | 29 |