FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-7R-DIV2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-7R-DIV2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17975017
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1662260.9247612950797209No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC445120.24763258916528424No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG268580.1494184956820903No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT233180.12972449483636095No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG213030.11851449153010536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA277850.050.0871774
GTACTCT278900.049.898613
TCTAGTT307850.045.03357
ACTCTAG349800.041.566145
TTGTACT336800.041.1868251
TGTACTC345750.040.3419342
CTCTAGT362600.039.4910166
TAGTTGT379600.036.3101469
CTAGTTG403850.034.103838
TGTTACC410450.033.41946813
GTTACCT410750.033.30114414
ACCTACT375100.032.9934733
ACACCTA375000.032.93712231
TACCTCT417550.032.77503616
CTAATGC419200.032.34464321
CACCTAC383850.032.2869632
ATGCTGG423350.031.82089224
TGGACAC420550.031.55855828
TCTAATG431300.031.49402820
TAATGCT439900.030.84640922
Produced by FastQC (version 0.11.5)