FastQCFastQC Report
Wed 28 Aug 2019
H3KMLBGXC_n01_0801-7G-MYB3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3KMLBGXC_n01_0801-7G-MYB3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14343870
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC1133320.7901075511699422TruSeq Adapter, Index 6 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT855260.5962547067144361No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC239770.1671585144037139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC155150.061.53975345
TATGCCG156400.061.00314746
GCACACG165000.060.9438611
CGTATGC156250.060.83771544
ACGTCTG169150.059.42711615
CACACGT172100.058.38955312
CTCGTAT153800.058.21130842
AGTCACG170750.058.1108228
TCGTATG160500.057.89654543
CACGTCT175050.057.5245214
AGCACAC175750.057.21614510
ACACGTC179500.056.1376213
GAGCACA184600.054.643769
GCCGTCT167500.054.32774749
TCCAGTC186500.053.4285425
CCAGTCA186400.053.38209526
CAGTCAC188000.052.9091627
CATCTCG164250.052.52604339
GACGACA184550.052.45673434
ACGACAT181300.051.27355635
Produced by FastQC (version 0.11.5)